【摘要】 用５～６ｄ的棉花无菌苗下胚轴切段与农杆菌共培，菌株质粒中Ｇｕｓ基因为标记基因，ＮＰＴⅡ基因为选择基因。在含０．１ｍｇ·Ｌ－１２，４－Ｄ和０．１ｍｇ·Ｌ－１ＫＴ的ＭＳ培养基上共培４８ｈ，转移到添加头孢霉素５００ｍｇ·Ｌ－１，卡那霉素５０ｍｇ·Ｌ－１的ＭＳ培养基中诱导和筛选抗性愈伤组织，７０～８０ｄ时，进行ＧＵＳ检测，选择ＧＵＳ阳性愈伤组织，经体细胞分化生成转基因再生植株。更多还原

【Abstract】 Hypocotyl segments from 5～6 day old seedlings of Gossypium hirsutum were cocultured with Agrobacterium tumefacience, GUS gene as marker gene, NPTⅡ as selecting gene. After the cultures were cocultured in MS medium contained 0.1mg·L12, 4D and 0.1mg·L1 KT for 48h, they were transferred into the medium described above, and suplemented with 500mg·L1 cefotaxime and 50～100mg·L1 kanamycin to induce and select resistant calli. After 70～80 days, GUS was numbered, tested and selected position, while calli were well cultured on embryonesis medium until globular embryos developed and germinated plantlets forming these embryos.更多还原