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大孔离子交换树脂分离纯化玻璃酸的研究
STUDY ON ISOLATION AND PURIFICATION OF HYALURONIC ACID BY MACROPOROUS ION-EXCHANGE RESIN CHROMATOGRAPHY
【摘要】 本文选用了四种离子交换树脂进行分离纯化玻璃酸的研究,结果表明,采用 D315大孔离子交换树脂,以去离子水为淋洗液, 0.6mol/L NaCl溶液为洗脱液,纯化效果较佳。纯化产品杂蛋白含量≤0.3%,回收率达71.1%,粘均分子量大于96万。
【Abstract】 The optimum purification’s conditions of HA by ion-exchange chromatography were studied. As a result, while macroporous ion-exchange resin D315 and 0.6mol/L NaCl solution as a eluent were selected. The purification efficiency was very well. The content of protein was less than 0.3%, the recovery was up to 71.1%, the average molecular weight of HA was more than 960,000.
【关键词】 玻璃酸;
大孔离子交换树脂;
分离纯化;
【Key words】 Hyaluronic acid; Macroporous ion exchange resin; Purification;
【Key words】 Hyaluronic acid; Macroporous ion exchange resin; Purification;
- 【文献出处】 离子交换与吸附 ,Ion Exchange and Adsorption , 编辑部邮箱 ,1998年04期
- 【分类号】TQ02
- 【被引频次】3
- 【下载频次】112