【摘要】 本文对亚急型、慢型克山病各1O例尸检心肌组织切片分别用CVB₃的cDNA探针作原位杂交和oncor公司的细胞凋亡原位检测试剂进行检测,结果8例亚急型克山病心肌上检出肠道病毒的RNA,同时8例亚急型克山病心肌上也检测出凋亡细胞,凋亡细胞核为棕褐色。10例慢型克山病心肌组织中有7例检出肠道病毒RNA,3例阴性,7例病毒RNA阳性的病例心肌上有6例检出凋亡细胞。3例未检出病毒RNA的病例有1例检出凋亡细胞,凋亡细胞检出的位置与肠道病毒RNA检出的位置基本一致。提示克山病的发生发展和肠道病毒感染高度相关,并有细胞凋亡机制参与。克山病的细胞凋亡可能由肠道病毒感染的诱导引起。更多还原

【Abstract】 This paper performed hybridization in situ to examine RNA of enterovirus infection in paraffin - embedded myocardial specimen from 10 patients with subacute Keshan disease and 10 patients with chronic Kenshan disease using the CVB3 cD-NA probe and apoptosis testing agent by Oncor corporation, Of the 10 patients with subacute tissues were infected by in-terovirus and had apoptosis. Of the 10 patients with chronic Keshan disease. It showed enterovirus infection and 6 of them had apoptosis, the rest 3 patients also had apoptosis. The apoptosis position is identical with the position of enterovirus infection. The results suggested that enterovirus infection may be highly related to Keshan disease and involved apoptosis.更多还原