【摘要】 通过基因工程操作，使乙型肝炎病毒ｅ抗原（ＨＢｅＡｇ）基因与绿色荧光蛋白基因（ＧＦＰ）融合，用新型Ｂａｃ－ｔｏ－Ｂａｃ杆状病毒表达系统在昆虫细胞中高效表达了ＨＢｅＡｇ－ＧＦＰ双功能融合蛋白。经ＥＬＩＳＡ法和荧光显微镜观察证实，表达产物既能发射易于检测的绿色荧光，又具有ＨＢＶ的ｅ抗原活性，为免疫诊断新方法的建立进行了有益的探索更多还原

【Abstract】 The chimeric gene of GFP and HBVe was transposed to shuttle vector Bacmid by Bac-to-Bac system and a bifunctional fusion protein was expressed in insect cells.The fusion protein was determined by ELISA Kit meanwhile the intensity of luminescence was observed under the fluorescent microscope.The results have demonstrated that the expressed GFP-HBVe fusion protein not only can generate striking green fluorescence but also have antigenic activity.The approach that the antigen was tagged by equal molecular green fluorescent protein allows us to use the bifunctional fusion protein to establish a new method for immunological diagnosis.更多还原