【摘要】 应用逆转录-多聚酶链反应及Sonthern杂交技术，研究卜内啡肽（β-END）对PHA诱导的人外周血单个核细胞IL－2和IFN－ΥmRNA表达的调节作用。结果发现β-END（10-8～10-14mol／L）可显著增强IL－2和IFN－ΥmRNA的表达并呈剂量依赖性关系，此外发现β-END对IL－2mRNA的增强作用可被阿片肽受体桔抗剂——纳络酮逆转。本研究从基因水平上证明了神经递质可通过影响免疫细胞细胞因子调节免疫功能。更多还原

【Abstract】 The effect of fyendorphin (β-END) stimulation of cytokines production by PHA-inducedhuman peripheral blood mononuclear cells (PBMC) was studied by reverse transcription incombination with polymerase chain reaction (RT/PCR), slot blotting and hybridization todetect and measure messenger RNA (mRNA). β-END significantly and dose dependently en-hanced IFN-Y and IL-2 mRNA expression of T cells and naloxone (Nal) reversed the effectof fyEND on IL-2 mRNA expression. The results further demonstrate the important link be-tween the neural system and the immune system.更多还原