【摘要】 通过免疫印迹鉴定,证明银杏(Ginkgo biloba L.)花粉中存在肌动蛋白。同时对银杏和玉米(Zeamays L.)花粉肌动蛋白含量进行了比较。结果表明,银杏花粉肌动蛋白含量明显少于玉米花粉肌动蛋白含量。SDS-PAGE扫描图谱显示,银杏花粉肌动蛋白含量只有玉米花粉的1/6。两种花粉DNaseⅠ活性抑制结果表明,银杏花粉肌动蛋白含量约为玉米花粉的1/7。更多还原

【Abstract】 Ginkgo ( Ginkgo biloba L.) is a famous gymnosperm species, originated from China and known as "living fossil" . Western blotting analysis has proved that actin also exists in ginkgo pollen like in angiosperm, but its content was only about one sixth as much as that of maize (Zea mays L,) pollen based on the densitometry of SDS polyacrylamide gel electrophoresis. Assay on actin inhibition of DNase I activity demonstrated that actin content of maize pollen was as about 7 times as that of ginkgo pollen. The plausible relationship between the actin content and the evolution of plant kingdom was discussed.更多还原