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几种抑制剂对钾和IAA诱导的离体黄瓜子叶不定根形成的影响
EFFECTS OF SOME INHIBITORS ON POTASSIUM- AND IAA-INDUCED ADVENTITIOUS ROOT FORMATION OF EXCISED CUCUMBER COTYLEDON
【摘要】 应用离体黄瓜(Cucumis sativus L.)子叶生根试法测定了几种抑制剂对钾和IAA诱导生根的影响。实验结果表明:7×10-4~7×10-1mmol/L的5-氟尿嘧啶和3.5×10-4~1.05×10-2mmoL/L的环已亚胺明显抑制钾和IAA诱导的离体黄瓜子叶的生根;0.1~1.0mmol/L的钒酸钠显著抑制钾和IAA诱导的离体黄瓜子叶的生根;2×10-4~2×10-1mmol/L的2,3,5-三碘苯甲酸也明显抑制钾和IAA诱导的离体黄瓜子叶的生根。钾诱导的生根与IAA诱导的生根密切相关,有可能钾对生根的促进作用是通过影响内源IAA的水平而实现的。
【Abstract】 The effects of some inhibitors on potassium- and IAA-induced rooting were studied adopting the root-formation bioassay in the excised cucumber ( Cucumis sativus L. ) cotyledon. 5-flu-orouracil at 7 × 10-4 - 7× 10-1 mmol/L and cycloheximide at 3.5 × 10-4 - 1.05 × 10-2 mmol/L significantly inhibited potassium- and IAA-induced adventitious root formation of the excised cucumber cotyledons, respectively .Na3VO4 at 0. 1 - 1.0 mmol/L obviously inhibited potassium- and IAA-induced adventitious rooting of the excised cucumber cotyledons, and similar inhibitory effect was found with 2,3,5-triiodobenzoic acid (TIBA) at 2×10-4-2×10-1 mmol/L. There is a close relationship between potassium- and IAA-induced adventitious rooting and the promotive effect of potassium on rooting is possibly brought about via affecting the endogenous level of IAA.
【Key words】 5-fluorouraeil; Cycloheximide. Na3VO4; 2. 3. 5-triiodobenzoic acid. Potassium; IAA; Excised cucumber cotyledon. Adventitious root formation;
- 【文献出处】 Acta Botanica Sinica ,植物学报(英文版) , 编辑部邮箱 ,1997年01期
- 【分类号】S642.2
- 【被引频次】16
- 【下载频次】163