【摘要】 目的 :观察 1991年 10月在中越边境广西凭祥购买的 1只野生食蟹猴 ( M4 )体内发现的疟原虫。方法 :将疟原虫血传给健康的切脾熊猴 ( M6) ,在猴体内的疟原虫配子体在 64个 /10 0 WBC时 ,给大劣按蚊叮咬感染 ,确定孢子增殖期 ,当蚊唾腺发现子孢子时 ,阳性蚊叮咬健康的熊猴 ( M5)后第 8d开始采血观察 ,确定红前期的时间。血内发现疟原虫后开始每 4 h采血 1次的定时观察 ,确定红内期裂体增殖周期及红内期各期疟原虫形态特征。结果 :该株猴疟原虫的孢子增殖期为 11d,红前期为 8d,红内期的裂体增殖周期为 4 8h。早晚期滋养体与人间日疟原虫形态相似 ,被晚期滋养体寄生的红细胞明显胀大 ,阿米巴样活动明显 ,可见薛氏小点 ,成熟的裂殖体内通常可见裂殖子 10— 15个。结论 :试验结果证实 ,该株猴疟原虫为食蟹猴疟原虫并定名为 CVH株 ( Plasmodiumcynomolgi CVH)。更多还原

【Abstract】 AIM:To observe the biological characteristics of a species of Plasmodium cynomolgi isolated from a wild- caught,naturally infected monkey Macaca irus(M4) at Pingxiang, Guangxi near the border between China and Vietnam in October 1 991 .METHODS:An un- infected splenectomized monkey(Macaca assamensis) M6 was inoculated iv with blood from M4.Nine days later,when M6 was found harboring gametocytes ata concentration of 6 4per 1 0 0 leucocytes,M6 was exposed to the bite of uninfected Anopheles dirus.On day1 4after the level of sporozoites in the salivary gland of An.dirus reached a high peak,the infected An.dirus was allowed to bite M5 ,a healthy,uninfected monkey(Macaca assamensis) .On day 8following infection,blood was taken from M5 twice a day and examined for malaria parasites.RESULTS:In terms of the appearance of a few rings in blood,the incubation peri- od is8days.At a temperature of about2 6℃ and a relative humidity of75 % ,oocyst reached maturity on day 1 1 ,and the sporogony was 1 1 days. The young trophozoites of Plasmodium cynomolgi were indistinguishable from those of Plasmodium vivax.The typical parasites were similar to other simian parasites of Plasmodium cynomolgi.There were Schüffner s dots in infected erythrocytes.The schizogony occurred every48hours.The ma- ture schizonts had a large number of merozoites,the number being1 0 - 1 5 .CONCLUSION: The malaria parasite described in the present paper was designated Plasmodium cynomolgi CVH(China,Vietnam and Huang) strain.更多还原