【摘要】 正常大豆Ｐｅｋｉｎｇ品种悬浮细胞具有典型的细胞结构，但经５００拉德γ射线照射后，细胞结构受到辐射损伤，表现为质体、线粒体等细胞器相对减少，核膜扭曲、模糊。但经１个月继代培养后，细胞恢复正常的结构和功能。５０００拉德γ射线能使大豆悬浮细胞产生致死损伤，导致细胞器解体和细胞死亡。本实验未能观察到辐射击穿细胞的直接证据，但出现膜渗透性改变、质壁分离及其内质网膨大等现象，间接证明了细胞自身防御体系被打破，有利于外源基因导入更多还原

【Abstract】 A Glycine max L cell suspension was used to study ultrastructural changes with irradiation treatment The cell was normal in cell ultrastructure with well-developed organs However, the number of mitochondrion per cell decreased and vacuoles increased 3 days after irradiation with 500 rad γ-Rays, cell structure changed dramatically with some cell organs disintegrated Although the damage was apparent, the cell was able to repair such damage within 1 month in culture and regained their normal appearance Treatment with 5000 rad γ-Rays brought about even greater change to the cell and caused the loss of growth ability The relationship between transformation rates and irradiation treatment is discussed更多还原