【摘要】 目的：观察ＥＧｂ及其所含成分槲皮素和内酯Ｂ对谷氨酸诱导的神经元损伤的拮抗作用．方法：对小鼠下丘脑弓状核作形态学观察和图象分析，采用ＭＴＴ法检测皮层原代培养神经元的活性，用Ｆｕｒａ２测定单个神经元内游离钙离子的浓度（［Ｃａ２＋］ｉ）．结果：ＥＧｂ及其内酯Ｂ能够保护神经元对抗谷氨酸的损伤，阻止谷氨酸诱发的［Ｃａ２＋］ｉ的升高．ＥＧｂ能够逆转谷氨酸诱导的下丘脑弓状核神经元核面积的减少．结论：ＥＧｂ及其内酯Ｂ可以通过降低［Ｃａ２＋］ｉ水平对抗谷氨酸神经毒性，内酯Ｂ很可能是ＥＧｂ中对抗谷氨酸神经毒性的主要成分之一．更多还原

【Abstract】 IM: To determine whether the extract of leaves of Ginkgo biloba L (EGb) and several active constituents of EGb have protective effects against glutamate (Glu) induced neuronal damage. METHODS: Microscopy and image analysis of nucleus areas in the arcuate nuclei (AN) of mice were made. The neuronal viability in primary cultures from mouse cerebral cortex was assessed using MTT [3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyl tetrazolium bromide] staining and the intracellular free calcium concentration ([Ca 2+ ] i) of single neuron was measured using Fura 2. RESULTS: EGb (2 5 mg·L -1 ) and its constituent ginkgolide B (Gin B, 2 mg·L -1 ) protected the neuronal viability against Glu induced injury, and prevented the Glu induced elevation in [Ca 2+ ] i. EGb (3 - 10 mg·kg -1 ) attenuated the decrease of nucleus areas in arcuate nuclei induced by Glu (1 g·kg -1 , sc). CONCLUSION: EGb and Gin B prevent neurons from Glu neurotoxicity through reduction of the rise in [Ca 2+ ] i.更多还原