【摘要】 由广宿主质粒ｐＲＫ４０４和微紫青霉外切葡聚糖纤维二糖水解酶Ｉ（ＣＢＨＩ）ｃＤＮＡ基因ｃｂｈＩ构建了重组质粒ｐＲＫ４０４－１８１，在携带有诱动质粒的菌株ＥＤ８６５４（ｐＲＫ２０１３）的存在下，采用三亲滤膜结合的方法，将该质粒转移到野油菜黄单胞菌Ｓ－１５２中，并成功地得到表达。结合子Ｓ－１５２（ｐＲＫ４０４－１８１）外切葡聚糖纤维二糖水解酶的ｐＮＰＣ活力比原始菌株Ｓ－１５２提高了２～４倍，滤纸酶活提高了１倍。更多还原

【Abstract】 Recombinant plasmid pRK404-181 was constructed with the wide-host-range plasmid pRK404 and the cellubiohydrolase I(cbh I) gene of Penicillium janthinellum. This plasmid was transferred into Xanthomonas campestris S-152 with the aid of helper plasmid pRK2013. As a result, the intracellular cellubiohydrolase activity of S-152 (pRK404-181) increased 2～4 time, and the filter paper degrading activity increased 1 time in comparison with the initial bacterial Xanthomonas campestris S-152. 更多还原