节点文献

Vero细胞法检测白喉抗毒素的研究

Vero Cell Assay for Determination of Diphtheria Antitoxin Titres

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 肖再平黄宏发王树巧谢广中

【Author】 Xiao Zaiping,Huang Hongfa,Wang Shuqiao,et al (Shanghai Institute of Biological Products,Shanghai 200052)

【机构】 卫生部上海生物制品研究所

【摘要】 参照Miyamura等报道,建立了微量细胞培养检测白喉抗毒素的方法。以家兔皮肤试验为参照,Vero细胞培养法敏感度为98.11%,特异度为84.00%,符合率为96.20%,相关系数r=0.93,在白喉血清抗毒素测定中值得推广

【Abstract】 Using the rabbit skin test as the control meliod,the in vitro vero cell assay method and the indirect hemagglutination test (IHA) were used to determine diphtheria antitoxin of 92 freshmen. The antitoxin titres obtained by IHA was compared with those determined by the rabbit skin test,the results confirmed a high correlation between Vero cell assay and rabbit skin test (r=0.93),the sensitivity of Vero cell assay was 98.11%,the accuracy was 96.20%,and the reproducibility was 92.39%. It is concluded that the Vero cell assay method is a highly recommended procedure for diphtheria antitoxin determination.

  • 【文献出处】 微生物学免疫学进展 ,PROGRESS IN MICROBIOLOGY AND IMMUNOLOGY , 编辑部邮箱 ,1997年01期
  • 【分类号】R392-33
  • 【下载频次】71
节点文献中: