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葡萄酒中游离氨基酸的高效液相色谱法测定
Determination of Free Amino Acids in Wines by Reversed Phase Liquid Chromatography
【摘要】 采用邻苯二甲醛衍生化法衍生葡萄酒中的游离氨基酸,以反相高效液相色谱法对葡萄酒中18种氨基酸进行了测定,方法简单、迅速,25min即可完成18种氨基酸的分离。精密度及回收率实验结果令人满意。
【Abstract】 Reversed phase liquid chromatography was employed to determine 18 free amino acids in wines with o-phthadialdehyde (OPA) as derivatizing reagent.The method is simple and rapid. 18 free amino acids were separated and determined in 25 minutes with satisfactory decision and recovery rate.
【关键词】 葡萄酒、氨基酸;
反相高效液相色谱法;
衍生化;
【Key words】 Wine; Amino acid; Reversed phase liquid chromatography Derivatizing reagent.;
【Key words】 Wine; Amino acid; Reversed phase liquid chromatography Derivatizing reagent.;
【基金】 山东省自然科学基金
- 【文献出处】 分析测试学报 ,JOURNAL OF INSTRUMENTAL ANALYSIS , 编辑部邮箱 ,1997年06期
- 【分类号】O657.7
- 【被引频次】16
- 【下载频次】351