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蚯蚓纤溶酶的糖成分分析

Composition Analysis of Sugar of the Earthworm Fibrinolytic Enzyme

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【作者】 张伟刘秀丽赵晓瑜

【Author】 Zhang Wei; Liu Xiuli; Zhao Xiaoyu(Biotechology reseach enter of Hebei University.Baoding 071002)

【机构】 河北大学生物技术研究中心!保定071002济南山东大学微生物系中科院武汉病毒所

【摘要】 以大豆胰蛋白酶抑制剂为配基的亲和层析制备的蚯蚓纤溶酶是一组糖蛋白。通过苯酚一硫酸法测中性糖,3,5一二硝基水扬酸法测还原糖,硅胶G薄层层析法鉴定糖的种类,结果为:蚯蚓纤溶酶主要含有中性己糖,含量为15%左右,TFMS的去糖处理会使蚯蚓纤溶酶酶活丢失.

【Abstract】 The earthworm fibrinolytic enzyme was prapared by affinity chromatography using soy been trypsin inhibitor as a ligand. It was a set of glycoproteins. The protocols used were phenol sulfuric acid method. 3, 5 -dinitrosalicylic acid method and thin-layer chromatography of silic gel G.1 was shown that the suger the enzyme contained was about 15% and mainly neutrol hexose.The enzyme was lost its activty after sugar being deleted with TFMS.

【关键词】 蚯蚓纤溶酶中性已糖糖蛋白
【Key words】 earthwormfibrinolytic enzymesugarglycoproteins
  • 【文献出处】 生物技术 ,BIOTECHNOLOGY , 编辑部邮箱 ,1997年04期
  • 【分类号】Q55
  • 【被引频次】23
  • 【下载频次】142
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