【摘要】 本文主要研究由病原徽生物引发的以婴幼儿为主的人的传染性腹泻，将其病原作为免疫原制备疫苗．对奶牛迸行人工主动兔疫后产生免疫乳。该免疫乳中免疫球蛋白（Ig）平均活性较非兔疫常乳高8倍，为初乳的14.84％。本试验就是使用由兔疫乳中提取的Ig，从致病性大肠杆菌和沙门氏菌中各选出一株致病性较强的菌株，作为病原菌代表株，即50155和44115。将Ig加入主细菌培养物中进行休外抑菌试验．在不同时间从培养物中取祥用751G分光光度计测定OD值，并用电子显徽镜迸行透射观察，最后将培养物再行接种，试验组经37℃24h培养后无菌生长。结果表明；每m1培养液中（此培养液在37℃24h时．活菌数达108cfu／ml）加入Ig1mg，耐病原菌的生长繁殖具有较强的抑制和杀灭作用。免疫乳中作为生物活性物质的成分，其抗菌效果较显著。更多还原

【Abstract】 The work mainly studied the infectious diarrhea caused by pathogenic microorganisms. The pathogens were treated as immunogens, and was developed into vaccines, which were used to immunize cows. The immuno—milk were then recovered and purified. The average activity of the immunoglobulins in the immuno—milk were 8 times higher than that of the non—immuno control, (14. 84% of the colostrum). One strong virulent strain of pathogenic E. coli 50155 and one strong virulent strain of Slmonella 44if5 were selected and used as the representative strains in the experiment.The bacteria inhibitory test as carried out in vitor with diffirent contents of the immunoglobulins, which were added to the bacteria samples harvested at diffirent incubation times. OD values were taken and bacteria were observed under electronic microscope. Finally. the bacteria were again inoculated and, no bacteria were obserred after 24 hours of incubation. Results showed that: when the immunoglobulins were added to the bacteria cultues (37℃, 24h, live bacteria count reached to 108CFU/ml) at the content of 1mg/ml. the bacteria inhibitory activity were relatively high. The antibacteria activity of the biological active components in the immuno—milk were significant.更多还原