【摘要】 本试验以火炬松、湿地松、晚松成熟种子的胚为材料，在附加有细胞分裂素（ＢＡＰ）的ＧＤ改良基本培养基（总盐分含量为０．２１％；总含氮量为１８６ｍｇ／Ｌ）经３～４周培养，在胚或子叶及下胚轴上出现膨大肉眼可见的暗绿色愈伤组织及小芽，其分化率达７５％～８５％。培养基中的细胞分裂素（ＢＡＰ）浓度对三种松种不定芽的分化具有很大影响，一般以３～５ｍｇ／Ｌ为好，在继代培养基中，活性炭对于嫩梢的生长具有良好的效果。当嫩梢长至１．５ｃｍ时，可供生根。在含有０．５ｍｇ／ＬＮＡＡ的生根培养基中经１５～２０ｄ培养，即长出白色愈伤组织，然后转至无激素培养基中培养１５～２０ｄ后生根。生根的小植株移栽入土壤，其成活率可达８０％～９５％。更多还原

【Abstract】 The embryos,of the mature seeds of P.taeda L., P.elliottii E., P.serotina M.,were used as experimental material. The explants were placed on modified GD medium(total salt 0.21%,total nitrogen 186 mg/L) suplemented with BAP for 3～4 weeks to induce swollen dark green callus,then the buds were found on embryos,cotyledon and hypocotyl.The rate of differentiation reached 75%～85%,and the concentration of BAP played an important part in the differentiation of adventiation buds of three pines,normally 3～5 mg/L is suitable.They were subcultured to medium containing activated charcoal,which is benefical to the growth of shoots.When the shoots grown to the length of 1.5 cm,they were planted on the rooting medium with 0.5 mg/L NAA for 15～20 days,and the white callus were visible.At this point they were transferred to the hormone free medium for 15～20 days to root,when the plantlets were planted in soil,85%～90% of them have survived.更多还原