【摘要】 采用甲基磺酸乙酯（ＥＭＳ）诱变草鱼吻端组织细胞（ＺＣ－７９０１）．用乌苯苷（Ｏｕａｂａｉｎ）筛选抗药性突变体．结果表明，ＥＭＳ对草鱼细胞的诱变效果与该药物对哺乳类细胞的诱变效果有明显差异．草鱼细胞对乌苯苷的敏感性也大大低于哺乳类细胞．ＺＣ－７９０１细胞在传代后２４ｈ，用浓度为２．８ｍｇ／ｍｌ的ＥＭＳ处理２４ｈ，诱变效果较理想，细胞杀伤率约３０％，这比通常用ＥＭＳ诱变哺乳类细胞所使用的浓度（０．４ｍｇ／ｍｌ）增大了７倍，存留下来的细胞用浓度为２５ｍｍｏｌ／Ｌ乌苯苷筛选抗性突变体，有少数细胞存活，并具有分裂增殖能力．但在细胞瓶内不易形成单层更多还原

【Abstract】 Mutants resistant to ouabain were selected while grass carp cell (ZC-7901) were treated with ethyl methanesulfonate (EMS). The results showed that there were distinct differences between fish cells and mammalian cells. Twenty four hours after they were subcultured then the grass carp cells had been treated with 2.8mg/ml EMS for 24h,and a good mutation effect was obtained. The cytolytic rate was about 30%. The concentration was 7 times higher than that for mammalian cells. Cells treated with EMS were used for selecting mutants with 25mM ouabain. Little cells were survived. They could repreduce. But confluent monolayer cells could not be developed in culture flasks.更多还原