【摘要】 本文为天蚕Antheraeyamamai丝素基因在家蚕Bombyxmori成功表达的首次报道。我们构建了天蚕丝素基因的YAC克隆，然后把含有该克隆的DNA溶液导入家蚕受精卵。分子杂交实验证明天蚕丝素基因已整合到家蚕基因组中。通过丝心蛋白氨基酸组分分析以及茧丝的溶解性比较，发现有部分转基因家委表达了天蚕丝素基因。F2代的转基因家蚕蛾的染色体DNA中同时还存在YAC序列，说明YAC对丝素基因具有介导作用。天蚕丝素基因以单拷贝形式存在于转基因家蚕中。更多还原

【Abstract】 This paper is the first report of the expression of Antheraea yamamai fibroin gene in Bombyx mori. We have constructed an yeast artificial chromosome (YAC) clone containing the fibroin gene of A. yamamai, and transferred this DNA clone into the fertil- ized eggs of B. mori. Dot blot indicates that the fibroin gene has integrated into the genome of B. mori. By analyzing the fibroin amino acid composition and the silk solubility, we found that A. yamamai fibroin gene has expressed in some of the transgenic B. mori. The existence of YAC sequence in the F, progeny of transgenic B. mori suggested that YAC is a medium for the transfer of fibroin gene. There is only one copy of A. yamamai firoin gene in the haploid of F, progeny.更多还原