【摘要】 ＳＰＦ试验鸡在８、２５日龄时接受了２次新城疫（ＮＤ）基础免疫，６０日龄时分别以标准新城疫病毒（ＮＤＶ）强毒株、中等毒力和弱毒疫苗株人工感染攻毒。应用单抗ＥＬＩＳＡ试剂盒，对所有试验鸡泄殖腔棉拭样品进行了３０ｄ的连续检测。结果表明：从攻毒后第３ｄ起强毒株试验组检测出多例阳性，检出高峰分别在攻毒后第４～６ｄ和１１～１５ｄ，与鸡群症状表现的时间相一致；中等毒力和弱毒疫苗株对照组均呈检测阴性。本研究证明单抗ＥＬＩＳＡ试剂盒可以检测免疫鸡群中ＮＤＶ强毒感染，为ＮＤ的监测提供了新手段。更多还原

【Abstract】 A ELISA kit was used to detect the infections of virulent Newcastle disease virus (NDV) in the experimentally immunized SPF chicken flocks. When cloacal samples from chickens infected with different pathotypes of NDV were assayed, only infections with virulent strains could be detected during the days 3-16 post challenge, while infections with commonly used mesogenic and lentogenic vaccine strains couldn’t be detected. The results in this presentation indicated that the ELISA kit could be used for detecting and monitoring virulent NDV infection in chicken flocks.更多还原