【摘要】 设计、合成了一种新的蛋白质特异性氧化断裂试剂BAPBE，用它共价修饰BSA的唯一疏基．修饰物与Fe2+螯合，在pH7．0、25℃下用H2O2及抗坏血酸钠处理，使BSA发生2种方式的断裂，产生4种BSA片段，但在相同条件下巯基枯草杆菌蛋白酶未被切断．更多还原

【Abstract】 A new protein cleaving reagent, 1-[4-(4-bromoacetylamidophenoxy)benzyl]EDTA (BAPBE), was synthesized from tyrosin in 7 steps. Only the mercapto group of BSA was alkylated with BAPBE and then the resulted modification, BSA-BAPBE, was treated with a solution containing Fe2+ and H2O2/ascorbic acid successively. It was found that BSA was cleft specifically in four fragments, their Mw were 5.7×103, 3.6×103, 3.0×103 and ≤103 respectively. Under the same condition, however, the thiol-subtilisin-BAPBE conjugate was unaffected.更多还原