【摘要】 研究了 36 5 nm波长紫外线辐射中国对虾精子对其顶体反应和受精能力的影响。结果表明 ,低剂量紫外线辐射促进精子发生顶体反应 ,大剂量辐射使精子丧失发生顶体反应的生理机能并死亡。 36 5 nm波长紫外线能够透过精荚和纳精囊盖 ,对其内精子起到杀伤作用。精液稀释液在紫外线下辐射 5～ 8s,仍有部分精子具有受精能力 ,但胚胎在早期阶段即死亡。人工诱导雌核发育的过程中 ,紫外线辐射精液稀释液 5～ 8s,可获得遗传物质失活的精子 (激活源 )。经透射电镜观察分析 ,紫外线对精子遗传物质的损伤是一种使染色质变性的化学作用。更多还原

【Abstract】 nm Ultraviolet (UV) light was used to study the effect in acrosome reaction and fertilization ability of UV irradiated Chinese shrimp sperm. The results are that, treatment of sperm with lower doses of UV irradiation successfully induced the acrosome reaction, however, the treated sperm lost their physiological function of acrosome reaction and died with high doses. 365nm UV can penetrate into the spermatophore and the thelycum crust to infure the sperm. Even a part of spermatozoa had fertilization ability with UV irradiated the sperm delution for 5 8 seconds, but the activated eggs died in the early stages of embryonic development and could not hatch. This study also showed that, during artifical gynogenetic development of Chinese shrimp, the method of 365nm UV irradiating the sperm dilution for 5 8 seconds could be used to get the genetic inactive sperm which called activation. The transmission electron microscope (TEM) analysis of UV irradiated shrimp sperm showed that the damage to the sperm was a kind of chemical change chromosomal denaturation.更多还原