【摘要】 采用十二烷基磺酸钠 -聚丙烯酰胺凝胶电泳 (SDS- PAGE)结合考马斯亮蓝染色和银染色法 ,鉴别口腔细菌。结果表明 ,同一菌株用不同菌体破碎方法 (SDS沸水浴破碎和超声波破碎法 ) ,不同批次处理的样品 ,不同次电泳、蛋白区带的不同染色法所得蛋白图型基本一致 ;而不同种的细菌电泳和扫描图型均不相同。提示 SDS- PAGE可用于菌种间鉴别 ,细菌属间鉴别较属内鉴别更能反映细菌亲缘关系的远近。更多还原

【Abstract】 Oral flora was classificated by SDS-PAGE combined with Comassie blue staining and silver staining. The results showed that each species of the test oral bacteria expressed its specific electrophorectic protein pattern, the way of sample preparation, staining did not change the protein pattern.This indicated that SDS PAGE could be used to classificate oral flora.The protein pattern was more obvious among the genus than that of within the genus.This showed that different relationship was among the bacteria. \;更多还原