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甲鱼爆发性传染病病原体分离及抗血清制备

ISOLATION OF PATHOGEN OF AN FULMINATING INFECTIOUS DISEASE OF SOFT-SHELLED TURTLE AND PRODUCTION OF THE ANTI-SERUM

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【作者】 史秋梅; 汤生玲; 高桂生; 马增军; 田在滋;

【Author】 Shi Qiumei Tang Shengling Gao GuishengMa Zengjun Tian Zaizi (Department of Animal Science, Hebei Agriculture Technique College)

【机构】 河北农技师院动科系; 河北农技师院动科系;

【摘要】 从患病甲鱼的肝脏中分离到9株细菌,其中7株为温和气单胞菌,2株为豚鼠气单胞菌。两种菌JA—1和JA—2对鲫鱼的半数致死量(LD50)各为4.3×105和5.5×107,在两种菌培养的上清液中均有一种具有溶血活性的毒素。研究表明:甲鱼的爆发性传染病是由温和气单胞菌和豚鼠气单胞菌引起,用两种菌为菌种做成菌苗,结合免疫增效剂免疫家兔制得血清,效价可达1:512,治愈率达100%。

【Abstract】 Nine strains of bacteria were isolated from the liver of the infected soft-shelled turtle. Among them, seven were Aeromonas sobria, the other two were Aeromonas caviac. The LD50 of the two strains, JA-1 and JA-2 to crucian carp were 4.3 ×105 and 5.5×107 respectively. There was a kind of hemolytic toxin in the supernatant of the two strains culture solution. The result showed that the fulminating infectious disease of soft-shelled turtle was caused by A. sobria and A. cariac,the titre of serum from rabbit immunited with the vaccine made from the two strains comban-ing with the immunity synergist could reach 1:512,and the cure rate was 100%.

【关键词】 甲鱼; 气单胞菌; 血清;
【Key words】 soft-shelled turtle Aeromonas serum;
  • 【文献出处】 河北渔业 ,Hebei Fzsheries , 编辑部邮箱 ,1997年06期
  • 【分类号】S947
  • 【下载频次】45
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