节点文献
抗菌素甲磺酸培氟沙星荧光分析法的研究
STUDY ON FLUORESCENCE METHOD OF PEFLOXACIN
【摘要】 甲磺酸培氟沙星是一种新型的抗菌素。本文对其荧光分析进行了研究。该物质在pH=3的三酸缓冲溶液中有很强的荧光发射光谱。线性范围较宽,检出限较低,在浓度为1×10-9~1×10-6mol/L间均成直线。同时,本文对甲磺酸培氟沙星在人血清和尿液中进行了回收测定,回收率在95~105%之间,相对标准偏差小于6%。
【Abstract】 Pefloxacin is a new kind of antibiotic.In this paper,a sensitive fluorescence method has been developed for the analysis of Pefloxacin.It has stronger fluorescence excitation.The fluorescence of Pefloxacin was measured at excitation wavelength 282nm and emission wavelength 440nm.The method is highly sensitive with lower limit of detection,calibration curves were linear between 1×10 -9 -1×10 -6 mol/L,with a regression coefficient of 0 9999.At the same time,recoveries of pefloxacin in Human serum Albumin and Urine are 95% and 105% respectively.
【关键词】 荧光法;
甲磺酸培氟沙星;
人血清;
三氯乙酸;
【Key words】 Fluorescene method; Pefloxacin; Human serum albumin; Urine (Received Oct. 23; 1996);
【Key words】 Fluorescene method; Pefloxacin; Human serum albumin; Urine (Received Oct. 23; 1996);
【基金】 山西省归国人员基金,山西省自然科学基金
- 【文献出处】 光谱学与光谱分析 ,SPECTROSCOPY AND SPECTRAL ANALYSIS , 编辑部邮箱 ,1997年02期
- 【分类号】O657.3
- 【被引频次】23
- 【下载频次】151