【摘要】 目的：探讨基因诊断在确定MF／SS受累淋巴结病变性质中的价值。方法：采用PCR-PAGE技术，研究了20例MF／SS受累淋巴结病变和10例良性皮病性淋巴结病变T淋巴细胞受体基因重排的特点。结果：检测出有TCR-β和／或TCR－γ基因克隆重排的：11／12例（91．7％）典型MF／SS淋巴结病变，5／8例（62．5％）临床病理可疑MF／SS或反应性淋巴结病变。10例良性皮病性淋巴结病变未检测出有TCR个或TCR－γ基因克隆重排，在8例临床病理可疑MF／SS或反应性淋巴结病变病人中，有TCR基因克隆重排的比没有重排的有明显短的生存期。结论：PCR－PAGE检测MF／SS受累淋巴结病变中T淋巴细胞受体基因重排，在其早期诊断及精确分期方面是相当有用的。更多还原

【Abstract】 To study the clinical value of molecular diagnosis in lymph nodes of the patientswith MF/SS. Methods:The polymerase chain reaction(PCR) technique was used to detect the clonal rearrangements of T-cell receptor (TCR) genes in 20 lymph nodes from patients with MF/SS and 10 dermatopathic lymph nodes from patients with benign skin disorders. Results:The dominant clonal TCR-βor γ gene rearrangements were detected in 11/12 (91. 7 % ) MF/SS lymph nodes, 5/8 (62. 5 % ) suspectedor uninvolved dermatopathic MF/SS lymph nodes. No dominant clonal TCR-β or γ gene rearrangementswere detected in 10 benign reactive dermatopathic control lymph nodes. In MF/SS patients with suspected or dermatopathic lymphadenopathy, patients with detectable clone T-cell populations had a significantly shorter survival than patients without such a population. Conclusion:This study demostratedthat the method is extremely useful for the early diagnosis and accurate stage in lymphadenopathy ofMF/SS.更多还原