【摘要】 ①目的研制人巨细胞病毒特异性转移因子（ＨＣＭＶ－ＳＴＦ），并试用于ＨＣＭＶ－ＩｇＭ和（或）ＩｇＡ阳性孕妇，观察其临床防治效果。②方法将ＨＣＭＶ－ＡＤ１６９株病毒感染人二倍体细胞，制备抗原，免疫成猪，待猪的免疫指标明显提高并稳定后，取脾及淋巴结，制备ＨＣＭＶ－ＳＴＦ，并用于治疗ＨＣＭＶ－ＩｇＭ和（或）ＩｇＡ阳性孕妇，采用间接ＥＬＩＳＡ法测血清ＨＣＭＶ－ＩｇＭ和ＩｇＡ，同时对羊水、脐血和婴儿尿做ＰＣＲ检测。③结果制备的ＨＣＭＶ－ＳＴＦ符合肌肉注射用药制剂标准，同时具有明显的促进ＬＡＣＡ小鼠脾细胞分化增殖效应。临床疗效验证表明，治疗组和对照组其血清ＩｇＭ及ＩｇＡ转阴率分别为７４．００％和６．６７％，两组比较差异有显著性（χ２＝１９１．１２，Ｐ＜０．０１）；治疗组和对照组先天性感染率分别为８．０４％和２２．２２％，两组比较差异有显著性（χ２＝９．９５，Ｐ＜０．０１）。④结论ＨＣＭＶ－ＳＴＦ对孕妇活动性巨细胞病毒感染及胎儿先天性感染有明显的防治作用。更多还原

【Abstract】 Objective To prepare human cytomegalovirus specific transfer factor(HCMV STF) and apply for the pregnant women with HCMV IgM and/or IgA positive in serum. Methods HCMV AD 169 staining was used to inoculate human diploid cells to obtain HCMV antigen which in turn was used to immunize adult pigs. When the immune indices of the pigs reached the required level, the HCMV STF was prepared from their spleens and lymph nodes and injected into the pregnant women actively infected with cytomegalovirus. The peripheral blood, amniotic fluid,umbilical blood of the pregnant women and the infants’ urine were collected before and after the injection respectively. HCMV IgM and IgA were tested with ELISA and HCMV DNA with PCR. Results HCMV STF thus prepared were in coordance with the standards for intramuscular injection and could enhance the proliferation of the spleen cells of LACA mice.Clinical practical study showed that the turning over to negativity of serum HCMV IgM and/or IgA was 74.00% and 6. 67% respectively in the therapeutic group and the control group and the difference was significant( χ 2=191.12, P <0.01). The congenital infection rate was also significantly different in these two groups( χ 2= 9.95, P < 0.01 ) Conclusion The preparation of HCMV STF obtained in this study has significant preventive and therapeutic effects for HCMV infected pregnant women and their embryoes.更多还原