【摘要】 对硬粒小麦（Ｔ．ｄｕｒｕｍ）－簇毛麦（Ｈ．ｖｉｌｏｓａ）杂种幼胚和Ｆ１幼穗诱导的愈伤组织进行秋水仙素处理获得硬－簇双二倍体ＴＨ１，ＴＨ１Ｗ，ＴＨ２Ｗ，ＴＨ３，ＴＨ３Ｗ。平均加倍率为７８．８％，最高为９４．１％。通过与普通小麦（Ｔ．ａｅｓｔｉｖｕｍ）回交，幼胚和花药培养，得到了６Ｄ／６Ｖ异代换系９４Ｇ２２－１，９４Ｇ２５－１，９４Ｇ３２－１，９４Ｇ３３－１。谷草转氨酶（ＧＯＴ）和酯酶（ＥＳＴ）同工酶可作为这些材料的生化标记。ＲＡＰＤ分析中，引物ＯＰＡＮ３可作为其分子标记引物更多还原

【Abstract】 During in vitro culture of young inflorescences of F 1 intergeneric hybrid from T.durum D311×H.villosa and immature embryo of intergeneric hybrid from T.durum Mexicali 75×H. villosa and T. durum 81086A×H.villosa, 742 calli were treated with colchicine. The results showed that the average rate of chromosome doubling was 78.8%. T. durum H.villosa amphidiploids TH1, TH1W, TH2W,TH3 and TH3W, were obtained. Homozygous materials 94G32 1,94G33 1,94G22 1 and 94G25 1,with the powdery mildew resistant gene from H.villosa were developed by backcrossing, immature embryo culture and anther culture. Glutamic oxalacetic transaminase and esterase could be used as the biochemical markers of the materials. In RAPD analysis, polymorphism could be found by using the primer OPAN3.更多还原