【摘要】 天花粉蛋白（ＴＣＳ）经羟胺特异性裂解成大小２个片断，应用兔网织红细胞破碎液蛋白翻译系统对其活性进行检测。研究发现ＨＡＴｆｌ（大片断）与完整天花粉一样能抑制上述系统的蛋白质合成，而ＨＡＴｆ２（小片断）则完全无此作用。结果提示天花粉蛋白的活性中心位于大片断部分。进一步的单抗封闭试验提示ＨＡＴｆ１与完整天花粉蛋白具有相似的反应格局。更多还原

【Abstract】 Trichosanthin(TCS) was specially cleaved by hydroxylamine to form two fragments,(HATf1 and HATf2). Using the rabbit reticulocyte lysate(a kind of protein trainslation system), detected their protein inhibition activity,the result indicated that HATf1,like TCS,also could inhibit the above system,but HATf2 had no this effect. All these results suggested that the active site of trichosanthin was locate on the HATf1. Blockage test of McAb(against TCS)indicated HATf1 had similar reactive pattern as TCS.更多还原