【摘要】 普萘洛尔和苄普地尔消除左甲状腺素诱发的大鼠心脏肥厚和线粒体Ｃａ２＋Ｍｇ２＋ＡＴＰ酶活力升高陈丁丁，戴德哉，陆坚，张新奎（中国药科大学药理研究室，南京２１０００９，中国关键词普萘洛尔；苄普地尔；左甲状腺素；心脏线粒体；Ｃａ２＋Ｍｇ２＋ＡＴＰ酶；心脏...更多还原

【Abstract】 IM: To study the effects of propranolol and bepridil on levothyroxine induced rat cardiac hypertrophy and mitochondrial Ca 2+ Mg 2+ ATPase activity elevation. METHODS: Rat heart hypertrophy was induced by ip levothyroxine 1 mg·kg -1 ·d -1 ×10 d. Then rats were treated by ig propranolol (Pro) or bepridil (Bep) 10 mg·kg -1 daily. Ca 2+ Mg 2+ ATPase activity and enzyme kinetic parameters were assayed. RESULTS: The activity and V max of mitochondrial Ca 2+ Mg 2+ ATPase isolated from hypertrophic left ventricle were 25±4 and 35 1±0 8 μmol P i·h -1 /mg protein, respectively, those of normal were 6 7±1 8 and 10±4 μmol P i·h -1 /mg protein, respectively. Apparent K m of the hypertrophic group Ca 2+ Mg 2+ ATPase was 0 4±0 12 mmol ·L -1 ATP, and that of normal was 0 59±0 22 mmol ·L -1 ATP. The total protein quantity of hypertrophic left ventricle was 80±30 mg, and that of normal was 47±9 mg. After treated with Pro or Bep (both 10 mg·kg -1 ig), the cardiac hypertrophy was attenuated, the enzyme activity and V max as well as total protein quantity of hypertrophic left ventricle were reduced to normal level, but apparent K m was not affected. CONCLUSION: Both Pro and Bep prevented the myocardium and its mitochondria from ischemia and overload calcium injury.更多还原