【摘要】 目的:观察金丝桃苷（Hpy）对缺血与再灌注心肌的保护作用及抗过氧化作用。方法:结扎家兔冠脉左降支60min后松开结扎20min,以多道生理记录仪持续记录左室内压（LVP）、左室内压变化速率（LV±dp/dt）、心电图（ECG）变化。再灌注后,取血和左心室肌测血浆肌酸磷酸激酶（CPK）,乳酸脱氢酶（LDH）及心肌阳离子含量。采用Langendorff系统,离体大鼠心脏缺血40min后再灌注30min。以荧光分光光度计测定冠脉流出液和心肌组织丙二醛（MDA）的含量。结果:Hyp10mg·kg^-1 iv可抑制缺血与再灌注所致的家兔LVP。LV±dp/dt_max、ECG、血浆CPK、LDH和心肌Ca²⁺、Mg²⁺、Na⁺含量的变化。Hyp10和100μmol·L^-1可降低缺血与再灌注所致离体大鼠心肌MDA含量的增高。结论:金丝桃苷对心肌缺血与再灌注具有保护作用,此作用可能与其抗脂质过氧化有关。更多还原

【Abstract】 AIM: To study the protective and antiperoxidative effects of hyperin (hyperoside; quercetin-3-O-galactoside; Hyp ) on myocardial ischemia/ reperfusion. METHODS: The rabbit anterior descenging branch of left coronary artery was occluded for 60 min and then released to allow reperfusion for 20 min. Hemodynamics (LVP, LV μp/df) and electrocardiogram (ECG, lead II) were monitored continuously with polygraph. After reperfusion, the blood sample and myocardium were taken to assay plasma creatine phosphokinase (CPK), lactate dehydrogenase (LDH), and cations in myocardium. Using a Langendorff system, the isolated heart of rat was initiated by ischemia for 40 min followed by 30 min of reperfusion. Malondialdehyde (MDA) contents of cardiac effluent and myocardium were measured with fluorescence spectrophotometer. RESULTS: Hyp 10 mg·kg-1 iv depressed changes in LVP, LV ± dp/dfmax, ECG, plasma CPK, LDH, and cations (Ca2+, Mg2+, Ma+) in myocardium induced by ischemia/reperfusion in rabbits. Hyp 10 and 100 μmol·L-1 markedly reduced the increase in MDA production in isolated rat hearts after ischemia/ reperfusion. CONCLUSION: Hyp possesses a protective effect against myocardial ischemia/ reperfusion injury via attenuating lipid peroxidation.更多还原