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鸡新城疫法氏囊病细胞组织培养灭活油乳化两联疫苗的研制

A STUDY ON COMBINED INACTIVATED OIL-EMULSION VACCINE AGAINST AVAIN ND AND IBD

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【作者】 刘洪云陈嘉棣杨惠萍杨颐施建春顾庆国孙凤萍徐卫林严仲烈许峰

【Author】 Liu Hongyun;Yang Huiping;Yang Yi;Shi Jianchun;Gu Qinguo;Seng Fengpin; XuWeilin;Yan Zhonglie;Xu Feng(Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 200030, China.)

【机构】 上海市农业科学院畜牧兽医研究所

【摘要】 采用细胞组织培养代替鸡胚培养的技术,分别对鸡新城疫细胞适应病毒株和本研究所分离鉴定的法氏囊细胞适应Q病毒株进行细胞组织培养,收获病毒,灭活,油乳化,制成联苗。经疫苗安全性、效力、免疫期和保存期测定,结果表明细胞组织培养联苗不仅安全,免疫率在92%以上,而且在保存期、免疫期超过半年以上时仍有坚强的免疫力,免疫效果理想。

【Abstract】 The NDV strain adapted to cell and IBDV strain Q isolated, identificated and also adapted to cell in our institute were propagated in vitro by cell culture. After inactivation, the high-titer fluids harvested from these propagations were made intocombined inactivated oil-emulsion vaccine against ND and IBD. The animal immunization test showed that the protection rate of the combined vaccine prepared in vitrocell culture was over ninety-two percent. The storage and efficacy tests proved its strong immune power and high quality.

  • 【文献出处】 上海农业学报 ,ACTA AGRICUL TURAE SHANGHAI , 编辑部邮箱 ,1996年01期
  • 【分类号】S859.797
  • 【被引频次】1
  • 【下载频次】84
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