【摘要】 暹罗鱼腥藻（Ａｎａｂａｅｎａｓｉａｍｅｍｓｉｓ）能代谢分子氢，其固氮酶和氢酶的放氢和吸氢均受其生长环境因子的影响。ＣＯ２对暹罗鱼腥藻之固氮酶的放氢和氢酶的放氢及吸氢显示不同程度的促进作用。在含５％ＣＯ２的空气条件下生长，藻细胞的氢酶放氢和吸氢活位分别为空气条件下的２．５倍和１．３倍；固氮酶的放氢活性为９６ｎｍｏｌＨ２ｍｇ－１ｃｈｌ－１ｈ－１，而在空气中生长的细胞则检测不出该活性，培养基中加１０ｍｍｏｌ／ＬＫＮＯ３和／或１０ｍｍｏｌ／ＬＮａＮＯ２，对其氢酶放氢活性影响不大，但其需氧吸氢和固氮酶的放氢均明显受到抑制。ＤＣＭＵ和Ｋ３Ｆｅ（ＣＮ）６等抑制剂对氢酶活性有不同程度的影响，一些金属离子对氢酶放氢有刺激作用，其中尤以Ｎｉ２＋和Ｍｏ２＋明显。更多还原

【Abstract】 The activities of hydrogenase and nidrogenase of Anabaena siamemsis were immediatly affected by the cell growth condition. With 5% of CO2 in air-gassing cultures,the activities of both H2-production and uptake of hydrogenase of whole cells were 2.5 and 1.3 times as high as those without CO2 respectivly. The hydrogen production of nitrogenase of the cells growing in 5% of CO2 in air was 96n mol H2 mg-1 chl-1 h-1,but this activity was not obeerved in air-grown cells. The activities of hydrogen uptake O2-dependent and nitrogenase were markedly inhibited by KNO2 and/or NaNO2 in the cultures,but no effect on hydrogen production of hydrogenase was obviously found. The different effects of some inhibitors as DCMU,CCCP and K3Fe(CN)6,and metal ions as Ni2+ and Mo2+ on hydrogen metabolism in A. siamemsis were described.更多还原