【摘要】 本文报道了一适宜人类急性髓系白血病祖细胞（AML-CFU）良好克隆性生长的无血清培养（SFC）体系。采用正交设计,得出主要血清替代物牛血清白蛋白（BSA）、饱和人转铁蛋白（STf）、胆固醇（Chol）、牛胰岛素（Ins）和牛氯化血红素（Hem）浓度依次为0.6％、2×10^-6M、2.8μg/ml、15μg/ml和0.06mM。重组人粒/巨噬细胞集落刺激因子（rhGM-CSF）和重组人白介素3（rhIL-3）在SFC体系中的种植率分别为0.776±0.621％和0.574±0.510％（（?）±s％）,与有血清培养（SCC）体系中的种植率无显著性差异（n=14,P>0.05）。更多还原

【Abstract】 The paper reports a SFC system supporting optimal clonal growth of human AML-CPU. Under the orthogonal design condition, the combination of the serum substitutes including 0.6% bovine serum albumin(BSA), 2×10-6M saturated human transfer-tin (STf), 2.8μg/ml cholesterol (Chol), 15μg/ml bovine insulin (Ins)and 0.06mM hemin (Hem) was critical inour SFC system. Moreover, in SFC the pkting efficiencies obtained by stimulating with recombmant human granulocyte/macrophage colony-stimulating factor (rhGM-CSF) andrecombinant human interleukin-3 (rhIL-3) were 0.776±0.621% and 0.574±0.510% (x+s%), respectively,which didn’t differ significanly from those in serum-containing culture (SCC) (n = 14, P>0.00).更多还原