【摘要】 本文测定了输精管可复性栓堵术（简称栓堵术）后精浆肉毒碱含量。精浆肉毒碱作为附睾的功能性指标早已被人们确认。肉毒碱是在微量酶反应系统下测定的。结果显示：栓堵术后随着时间的延长精子完全消失率增加；栓堵术前精浆肉毒碱浓度平均为３３６．９±７８．１ｎｍｏｌ／ｍｌ（ｘ±ｓｎ＝５８），栓堵术后精子完全消失者和未完全消失者精浆肉毒碱浓度分别为１１２．７±５０．７ｎｍｏｌ／ｍｌ和１７２．５±７１．７ｎｍｏｌ／ｍｌ，明显低于栓堵术前（ｎ＝１７２和ｎ＝５１，Ｐ＜０．０１），精子完全消失者精浆肉毒碱浓度比未完全消失者明显降低（Ｐ＜０．０１）。栓堵术后有３例精液常规检查精子计数＞２．０×１０６／ｍｌ，精浆肉毒碱含量都在正常范围（术前）内。提示栓堵术后附睾分泌产生的物质有可能渗透到精液中抑或通过栓子与输精管壁间的空隙流出，栓堵术后精浆肉毒碱的含量可作为判定输精管栓堵是否完全或成功的一个辅助指标。更多还原

【Abstract】 Reversible injection occulsion of vas deferens (RIOVD)is a safe,effective and simple method of male fertility control. Carnitine was determined by micro-enzymatic method.The incidence of sperm disappearance increased with the duration of RIOVD and reached 90% at the end of 12 months after operation.Before RIOVD,the mean value of carnitine in seminal plasma was 336.9±78.1 nmol/ml(x±s,n=58);after RIOVD,the mean value of seminal plasma carnitine was 112.7±50.7 nmol/ml(n=172) in the group with sperm disappearance,and 172.5±71.7nmol/ml(n=51)in the group without sperm disappearance.There was a significant difference in carnitine content between pre-operation and post-operation(P<0.01).After RIOVD the carnitine concentration in seminal plasma of the group with sperm disappearance was lower than that of the group without sperm disappearance(P<0.01).The results suggest that carnitine content in seminal plasma was a reference index for judging the success or failure of the operation.更多还原