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B链氨端去-肽(B1)羧端去五肽(B26—30)猪胰岛素的初步晶体学研究
THE PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF Des-B1-DESPENTAPEPTIDE(B26-30)INSULIN
【摘要】 应用悬滴气相扩散法在含6%氯化纳和12.5%丙酮的柠檬酸缓冲体系中,获得可供X射线结构分析用的B链氨端去一肽(B1)羧端去五肽(B26—30)猪胰岛素(DesB1-DPI)单晶体。晶体属四方晶系,空间群为P4122或P4322,晶胞参数为:a=b=36.0A,c=120.0A,a=β=γ=90°。单位晶胞中每个结晶学不对称单位含有2个DesB1—DPI分子。
【Abstract】 Single crystals of DesB1-Despentapeptide (B26-30) Insulin (DesB1-DPI) suitable for X-ray diffraction analysis have ho grown in citrate, acetone and sodium chloride system by hanging drops vapour diffusion method. The crystals belong to tetragonal system with space group P4122 or P4322. The parameters of the unit Cell are : a=b = 36.0A, c= 120.0A, α=β=γ= 90°. There are two DesB1-DPI moleCUles in an asymmetric unit.
【关键词】 胰岛素;
DesB1—DPI;
单晶体;
晶体结构;
X射线衍射;
【Key words】 Insulin; DesB1-DPI; Single crystal; Crystal structure; X- ray diffraction;
【Key words】 Insulin; DesB1-DPI; Single crystal; Crystal structure; X- ray diffraction;
【基金】 国家自然科学基金,科学院重大项目基金
- 【文献出处】 生物物理学报 ,ACTA BIOPHYSICA SINICA , 编辑部邮箱 ,1995年01期
- 【分类号】Q578
- 【下载频次】24