【摘要】 本文观察了小鼠接种高分泌ＴＮＦ－ａ的Ｂ１６黑色素瘤细胞（Ｂ１６－ＴＮＦ－ａ￣＋）后体内免疫细胞（包括ＣＴＬ、ＮＫ细胞、ＬＡＫ细胞以及腹腔ＭΦ）杀伤活性的变化。实验结果发现：接种Ｂ１６－ＴＮＦ－ａ￣＋后第１５天，小鼠脾细胞ＮＫ活性和诱导后的ＬＡＫ活性显著高于对照组，而经诱导后的ＣＴＬ杀伤活性则无显著变化；腹腔内巨噬细胞吞噬功能及杀伤活性也显著提高，但其Ｉａ抗原表达与对照组无显著差异。本实验的结果表明：高分泌ＴＮＦ－ａ的黑色素瘤细胞主要诱导并增强机体非特异性免疫细胞的抗肿瘤作用。更多还原

【Abstract】 The in vivo induction of the cytotoxicity of killer cells by TNF-agene-transfected tumor cells(B16-TNF-a￣+) was studied. Fifteen davs after the inoculation with thexetumor cells, the NK activity of thesplenocytes and the LAK activity induced from the splenocytes increased significantly, but the cytotoxicity ofCTL did not.In addition,the cytotoxicity and phagocytotic ability of the peritoneal MΦfrom mice inocula-tion with B16-TNF-a￣+ cells also increased significantly;however,the expression of the MHC Ia on MΦdidnot change.These results demonstrated that the in vivo cytotoxicity of the NK cells, LAK and peritonealMrp may be augmented by inoculating the mice with TNF-agenetransfected tumor cells.更多还原