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高灵敏度人促甲状腺素(TSH)免疫放射分析法
A SENSITIVE IMMUNORA-DIOMETRIC ASSAY OF THYROPIN(TSH)
【摘要】 用两种抗人促甲状腺紊(TSH)分子不同抗原决定簇,彼此互不干扰的单克隆抗体(McAb)建立了TSH一步双位夹心,固相免疫放射分析法(IRMA)简便快速、不需离心、灵敏度高、(0.02Iμ/ml)特异性强。
【Abstract】 e describe a twosite-immunoradiometric assay for thyrotropin(TSH)in serum,based onuse of two monoclonal antibodies directed against two separate antigenic determinants on theTSH molecule. One antibody is immobilized on polystyrene beads,the other is radioiodinated bya modified chloramine T method. The detection limit of the assay is 0. 02 milli-int U/L. Theworking range is from 0. 15 to 150 mill-int.U/L. The normal range 0.36;0 milli-int.U/L.
- 【文献出处】 中国免疫学杂志 ,CHINESE JOURNAL OF IMMUNOLOGY , 编辑部邮箱 ,1994年04期
- 【分类号】R446.6
- 【被引频次】3
- 【下载频次】53