【摘要】 将人酸性纤维母细胞生长因子’（ａＦＧＦ’）ｃＤＮＡ的ＰＣＲ产物以ＴＡ连接方式克隆入ｐＣＲ￣（ＴＭ）ＩＩ质粒，然后采用Ｔ７和Ｓｐ６启动子特异性引物对克隆的片段以双脱氧未端终止法进行双链ＤＮＡ测序。结果表明这项技术是一种快捷而可靠的克隆及分析ＰＣＲ产物的方法更多还原

【Abstract】 Human acidic fibroblast growth factor’cDNA amplified in PCR was cloned into pCR￣(TM) IIplasmid by TA Iigation and sequenced directly by using dideoxynucleoside chain termination re-action primed by T7 and Sp6 primers. The results demonstrated that the technique was a rapidand relitable method for cloning and sequening PCR products.更多还原