【摘要】 本文在研究了人白细胞介素6(IL-6)基因转染瘤苗对白血病小鼠体内治疗作用及其部分免疫机理的基础上,进一步观察了IL-6基因转染瘤苗治疗白血病小鼠后产生细胞因子(包括IL-1,IL-2,IFN-γ,TNF及GM-CSF)水平的变化。结果表明,IL-6基因转染瘤苗在体内对小鼠脾淋巴细胞或腹腔巨噬细胞(Mφ)经诱导后分泌的上述细胞因子(除IFN-γ外)各组均有不同程度的促进作用。以此种新型瘤苗辅以低剂量IL-2和低剂量环磷酰胺(Cy)治疗后促进作用最为明显。从而为IL-6基因转染瘤苗治疗白血病的免疫学机理提出了新的认识。更多还原

【Abstract】 In the present study, we have further investigated the effect of IL-6 gene-transfected leukemia cellular vaccines on the production of cytokines ( IL-2, IFN-γ, TNF and GM-CSF) induced from the splenocytes or peritoneal macrophages of the leukemia-bearing mice. The results demonstrated that in vivo adminstration of IL-6 gene-transfected vaccine might increase the induction of most cytokines, except for IFN-γ, from the splenocytes or peritoneal macrophages. The enhancing effect was most obvious when IL-6 gene-transfected leukemia cellular vaccine was used in combination with low’dose of IL-2 and low dose of cyclophosphamide. From these results we have further insight into the immunological mechanisms for the application of this new type of leukemia cellular vaccines in the leukemia biotherapy.更多还原