【摘要】 本文系探讨ｄｏｔ－ＥＬＩＳＡ是否单一检测循环抗原。结果显示，正常人血清及正常兔血清的各分离部分均呈阴性反应，血吸虫病患者血清及感染兔血清经三氯醋酸处理的上清液，及经聚乙二醇处理的沉淀物均出现阳性反应。表明ｄｏｔ－ＥＬＩＳＡ不仅检测循环抗原，而且同时检测免疫复合物。更多还原

【Abstract】 Serum samples were collected from normal rabbits,normal persons, Schistosoma japo nicum infected rabbits and human cases respectively,and were treated by TCA precipitation and PEG precipitation,and subsequently detected by dot ELISA.The results showed that both the supernatants of TCA treated antisera from infected human cases and rabbits and the precipitants of PEG treated antisera of the same origins showed positive reactions,while no positive reactions were found for sera from normal rabbits and normal persons.It was exhi bited that dot ELISA detected not only the circulating antigen of schistosomes but also the immunocomplex.更多还原