【摘要】 应用放射受体分析法对肝性脑病（ＨＥ）及对照大白鼠脑粗制突触体膜（Ｐ２膜）的血管活性肠肽（ＶＩＰ）受体结合特性进行了测定。结果：ＨＥ鼠最大结合容量（Ｂｍａｘ）为９．５６±０．２９ｆｍｏｌ／ｍｇ蛋白质，平衡解离常数（ｋｄ）为０．２８±０．０１ｎｍｏｌ／Ｌ，较对照鼠Ｂｍａｘ值明显减少（Ｐ＜０．００２），而ｋｄ值差异无显著意义（ｐ＞０．２０）。表明ＨＥ较对照鼠脑Ｐ２膜的ＶＩＰ受体结合位点数量减少，但亲和力不变；提示其在ＨＥ发生中具有一定作用。更多还原

【Abstract】 e evaluated the effect of vasoactive intestinalpeptide (VIP) receptor, a brain-gut peptide receptor,which is capable of exciting central neurons, on thepathogenesis of hepatic encephalopathy(HE). Bymeans of radioligand binding assay, VIP receptors incrude synaptosomal membrane of rat brains were in-vestigated in a rat modei of HE induced by partialhepatectomy following carbon tetrachloride intoxica-tion and in controls. A dissociation constant (Kd) wasfound 0.28 ± 0.0lnmol / L and a maximal binding ca-pacity (Bmax) 9.56 ± 0.29fmol / mg of protein in HErats. Only decreased Bmax values were observed(P<0.002) and the Kd values were statisticallyunchanged (P> 0.20) in HE rats as compared with incontrols. The results suggest that the changes of VIPreceptors in brains play a significant role in thepathogenesis of HE. The mechanism of HE indueedby the alterations of VIP receptors in the brains wasdescribed.更多还原