【摘要】 通过免疫沉淀法检测c-fos蛋白,研究辛卡利特和和阿片肽对大鼠脑和脊髓c-fos表达的影响。结果表明,辛卡利特和NDAP可刺激脑和脊髓c-fos的表达(P均<0.01),羟甲芬太尼也有诱导作用(P<0.01)。而辛卡利特和NDAP对脊髓c-fos的表达有协同作用(P<0.01),相反,羟甲芬太尼则拮抗辛卡利特对脑和脊髓c-fos的表达(P<0.05)。结果提示在c-fos水平辛卡利特与不同的阿片肽起不同作用。更多还原

【Abstract】 Sincalide (CCK-8) is an antiopi-oid substance. In this study, we investigated the effects of sincalide and opioids on c-fos expression and their interaction in brain and spinal dorsal horn in vitro. Immunoprecip-itation was used for detection of c-fos protein. The results indicated that 0. 1 μmol ·L-1 sincalide induced c-fos expression markedly in both brain (a 3. 8-fold increase in c-fos protein level) and in spinal dorsal horn (a 3. 6-fold increase). NDAP (a K receptor agonist) 0.1 μmol ·L-1 showed some activating effects on c-fos expression, the c-fos protein level increased 2. 7 and 2. 6 times respectively in brain and spinal dorsal horn. Ohmefentanyl (Ohm, a μ. receptor agonist) 0. 1 μmol·L-1 al-so exhibited an inducing effect on c-fos protein production. Sincalide and NDAP exerted some additive effects on c-fos protein production in spinal cord. In contrast, the effect of sincalide on c-fos protein production is antagonistic to that of Ohm. The results suggested that there were changing patterns of interaction on c-fos expression between sincalide and opioids.更多还原