【摘要】 本文采用整体犬急性心肌缺血－再灌注模型以探讨穿心莲提取液减轻钙超负荷的机制。结扎冠脉左前降支上１／３处９０ｍｉｎ后再灌注６０ｍｉｎ较持续结扎１５０ｍｉｎ，缺血中心区心肌细胞内Ｃａ２＋增加（Ｐ＜０．０５）、Ｎａ＋明显增加（Ｐ＜０．０１），心肌细胞膜Ｃａ２＋－ＡＴＰａｓｅ活性明显降低（Ｐ＜０．０１），心肌组织ＭＤＡ含量显著增高（Ｐ＜０．０１）。而在再灌注前４５ｍｉｎ静脉给予穿心莲提取液，则见其缺血中心区心肌细胞内Ｃａ２＋降低（Ｐ＜０．０５）、Ｎａ＋明显降低（Ｐ＜０．０１），心肌细胞膜Ｎａ＋－Ｋ＋－ＡＴＰａｓｅ、Ｃａ２＋－ＡＴＰａｓｅ活性明显增加（Ｐ＜０．０１）。心肌组织ＭＤＡ含量显著降低（Ｐ＜０．０１）。说明穿心莲提取液减轻心肌缺血－再灌注过程中钙超负荷与减轻氧自由基危害、保护心肌细胞膜ＡＴＰａｓｅ活性、降低钠超负荷有关。更多还原

【Abstract】 Mechanism of APN in alleviating the Ca￣2+-overloading in dog model during the process of ischemic reperfusion was studied.In comparison with the sustained ischemic group,the parameters in reperfusion ischemic group showed:In central ischemic region of myocardial cell Ca￣2+ increased （P<0.05）,Na￣+ increased markedly （P<0.01）,Ca￣2+-ATPase activity dropped markedly （P<0.01）,MDA increased significantly （P<0.01）,whereas in the APN pretreated group,Ca￣2+ in relevant area reduced（P<0.05）,Na￣+ decreased significantly（P<0.01）,and the activity of Ca￣2+-ATPase and Na￣+-ATPase increased markedly（P<0.01）,MDA decreased significantly（P<0.01）.These data indicate that APN may improve the activity of Sarcolemma ATPase in alleviating the Ca￣2+ and Na￣+-overloading by decreasing the harmful effect of O₂ free radicals.更多还原