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应用ABC-ELISA和SPA-ELISA检测狂犬病疫苗免疫犬后血清中的抗体水平

DETECTION OF ANTIBODIES FROM DOGS VACCINATED WITH RABIES VACCINE BY ABC-ELISA AND SPA-ELISA

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【作者】 郭万柱曹军孔树全冯炳芳蒋文灿

【Author】 Guo Wanzhu et al(College of Animal Science and Technology Sichuan Agricultural University, Yaan,Sichuan, China 625014)

【机构】 四川农业大学动物科技学院

【摘要】 本文报道应用ABC-ELISA和SPA-ELISA检测狂犬病疫苗免疫犬后血清中抗体的效价,比较研究了两种方法的特异性,敏感性和重复性,结果显示两种方法均适用于检测狂犬病血清抗体水平。ABC-ELISA较SPA-ELISA敏感性高4倍,前者的阳性检出率(100%)高于后者(92.8%)。

【Abstract】 This paper reported detection of antibodies from dogs vaccinated with rabies vaccine by ABC-ELISA and SPA-ELISA,Thespecifiaty, sensitivity and stability of both mothods were compared.The results showed that both methods were applicable todetccting the level of antibody to rabies virus. The sensitivity of ABC-ELISA was four times as much as that of SPA-ELSA. Thepositive rate (100%) in ABC-ELISA was higher than that (92.8%)in SPA-ELISA.

  • 【文献出处】 四川农业大学学报 ,JOURNAL OF SICHUAN AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1994年02期
  • 【分类号】S852.4
  • 【被引频次】2
  • 【下载频次】53
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