【摘要】 用固相亚磷酰胺法合成了家畜和家禽的促黄体素释放激素基因的３个寡核昔酸（Ｌ１、Ｌ２和Ｌ３）片段。经退火，聚合成平端双链后，以ＢａｍＨＩ和ＨｉｎｄⅢ双酶切，插入载体ｐＢｌｕｅｓｃｒｉｐｔ的多克隆位点中，通过菌落原位杂交、酶切位点分析及聚丙烯酞胺凝胶电泳鉴定，证实化学合成的促黄体素释放激素基因已在Ｅ．ｃｏｌｉＤＨ５ａ中克隆成功，序列分析结果表明人工合成的基因序列完全正确。更多还原

【Abstract】 The genes coding for mammal and avian luteinizing hormone releasing hormone(LHRH)havebeen chemically sythesized by solid-phase phosphoramidite methed. The 51 base-pair synthetic DNA duplexconsists of structural gene encoding LHRH,two stop codon TAA,TGA and Hind Ⅲ restriction site at 3’end and BamHI restriction site at 5’end of gene. The recombinant colonies were identified by in situ hy-bridization and restriction enzyme digestion,Their accuracy was comfirmed by DNA Sequence Analysis.更多还原