【摘要】 研究结果表明，家鼠型ＥＨＦＶ台４１第四代细胞传代株在沙鼠肾原代细胞上测得病毒ＴＣＩＤ＿（５０）为１０￣（６．５）／ｍｌ，血凝素滴度达１：５１２．影响血凝素滴度的主要因素有单层细胞的密度，病毒的接种量，血凝素收获的时间和血凝的最适ｐＨ值。更多还原

【Abstract】 The result of study revealed that the viral TC ID₅₀ was 10￣（6.5）/ml andthe itre of hemoagglutinin （HA） reached 1: 512.which had been examined on Meriornes Unguiculatus Kidney primary cells of the forth gener-ation cell transmitted strain of house-rat type EHFV T₄₁ These two major index meet the national require-ments of EHF testing inactivated vaccines for the clinical observation. EHFV T₄₁ viral strains,isolated frompatient’s peripheral blood, were not transmitted in the continuos cell lines and no potential carcinogenic fac-tors existed.The viral strains can be used to prepare high-titre HA but also act as spare viral strains forpreparing house-rat type EHF vaccines,It was also observed that the density of monolayer cell,inoculatingviral amount,gaining time of HA and proper PH value of HA are main factors to influence the HA titres.更多还原