【摘要】 用卫氏并殖吸虫囊蚴感染大白鼠,检获两种滞育虫体,早期滞育虫体和晚期滞育虫体,用聚丙烯酰胺等电聚焦技术对两种滞育虫体的苹果酸脱氢酶(MDH)的同工酶酶谱进行分析,并与犬体童虫和成虫的酶谱相比较,结果发现在鼠体内从早期滞育虫体到晚期滞育虫体MDH同工酶区带增多,符合“卫氏并殖吸虫的MDH同工酶区带随发育呈增多及复杂的趋势”的研究结果。鼠体的晚期滞育虫体与犬体童虫和成虫的MDH酶谱基本相似,提示卫氏并殖吸虫在转续宿主体内滞育虫体的MDH同工酶的基因表达及修饰过程与适宜宿主体内的虫体可能一致。更多还原

【Abstract】 In this research program, two kinds of diapause worms, from rats infected by metacercariae of Paragonimus westermani (Kerbert, 1878) were applied i. e. early diapause worms and later diapause worms. The malate dehydrogenase (MDH) isozyme patterns of the two kinds of diapause worms were determined with isoelectrofocusing electrophoresis (IEF) in polyacrylamide gel and were compared with that of the adolescent worm and the adult from the normal host (dog). It showed that the number of isozyme bands increased from the early diapause worm to the later diapause. It also showed that the pattern of the later diapause worm was similar to one of the adolescent worm and the adult from dog. That indicated that the method of MDH isozyme gene expression and repairmeht in P. westermani diapause worm may be possible to be the same as the worm in the normal host.更多还原