【摘要】 本文应用PCR技术从人基因组DNA中扩增出胰岛素基因片段（1128bp）,将扩增产物直接克隆到M₁₃载体中去,用双脱氧链终止法作DNA序列分析。是鉴定胰岛素基因中前肽、A链、B链和C肽编码区域有无碱基突变的一种简便、有效的方法更多还原

【Abstract】 The polymerase chain reaction(PCR) was used to amplify insulin gene fragment (1128bp) in human genomic DNA. The amplified product was directly cloned to MI3 vector, and DNA sequencing was carried out by Sanger dideoxy-mediated chain termination method. This is a simple and effective method for identifying mutations in the coding region of prepeptide, A chain, B chain and C peptide of insulin gene更多还原