【摘要】 把已经消毒的滤纸、垫料、工作用白大衣碎片、固型饲料浸透于实验动物饲养场所培养出来的2株（N022、N043）稀释为10^?的需氧芽胞杆菌液中,同时也滴在不锈钢片上,待其自然干燥。然后用相对湿度90％和一定浓度的臭氧进行熏蒸。实验结果,除固型饲料以外,都认为对滤纸、垫料、工作用的白大衣碎片在臭氧的浓度600ppm和6h的处理后都能够杀菌。但是固型饲料内的芽胞在浓度500ppm～1000ppm的6h或200ppm的24h臭氧的处理,仍然不能杀灭芽胞。其原因可能受到共同存在饲料中的蛋白质的影响所发生的障碍有关。同时本实验为了证明臭氧对动物有否影响,使用60只4周龄,雌性ICR/Jcl小鼠（日本CLEA）进行了臭氧的安全性实验研究,经过24h,一个月,二个月的实验后。实验组和对照组在体重增加、摄食量、摄水量及病理学上均未见异常。更多还原

【Abstract】 2 Strains of B. licheniformis isolated from laboratory animals were examined for resistance to ozoae fumigation. Phosphate-buffered saline containing 108 Bacillus spores was dropped onto sterilized filter strips, woodchip bedding, cotton cloth pieces, pellets of diet and stainless steel plates. After drying at room temperature, the test materials were exposed to ozone gas of different concentrations at 90% relative humidity. Exposure to 200ppm ozone for 6 hours was sufficient to kill spores in filter strips, while a little higher concentration or a little longer period of ozone fumigation was necessary for sterilization of wood chips, cotton cloth pieces and steel plates. However, exposure to ozone gas of 500-1,000ppm for 6 hours or 200ppm for 24 hours could not kill spores in pellets of diet, suggesting that dietary protein inhibited the bactericidal activity of ozone. The present results indicated that 600ppm ozone fumigation for 6 hours might be effective for routine sterilization of cages, bedding, working clothes and other materials used in laboratory animal facilities except for pellets of diet.we also examined residual toxicity of ozone gas. Male ICR mice were kept for 3 months using cages, bedding and filter caps which had been sterilized by 600ppm ozone fumigation for 6 hours, and drinking water which had been sterilized by 200ppm ozone fumigation (ozone-exposed group). In the control group, ICR mice were kept using the equipments and drinking water which had been sterilized by autoclaving. The equipments and drinking water were changed twice a week. Body weight, food and water consumption of the mice ( 10 mice for each group) were scored 24 hours, 1 month and 3 months after the initiation of th3 experiment. The mice were also examined histopathologically. Body weight gain, food and water consumption of the mice were not significantly different between ozone-exposed and control groups. No pathological abnormalities were observed in the mice kept in the ozone-~sterilized materials.更多还原