【摘要】 鳗鲡卵黄蛋白制备物与血清在SDS-聚丙烯酰胺凝胶电泳上分别呈现出2条相对应的蛋白区带,迁移率（R_f）相同。对照组雌鳗经5次和7次注射HCG与CPG后,成熟系数（GSI）分别为（11.94±2.83）%和（46.04±6.22）%,血清中17β-雌二醇（17β-E₂）浓度为（0.665±0.431）ng/ml和（0.421±0.010）ng/ml,睾酮（T）为（4.603±3.442）ng/ml和（3.817±1.399）ng/ml,经HCG.CPG与鳗鲡卵黄蛋白三者混合注射5次和7次后,GSI分别为（16.53±6.67）%和（53.23±11.25）%,血清中17β-E₂浓度为（1.036±0.316）ng/ml和（0.411±0.068）ng/ml,T为（11.170±5.818）ng/ml和（3.956±1.780）ng/ml。未经注射催情药物的雌鳗GSI为（0.50±0.32）%,血清中17β-E₂和T浓度低,检测不出。试验结果证明,外源性鳗鲡卵黄蛋白与HCG和CPG三者混合注射具有明显的促进鳗鲡卵巢加速发育成熟的作用。更多还原

【Abstract】 Both yolk protein preparation （YPP） and serum of Japanese eel （An-guilla japonica） showed two bands on SDS-gel electrophoresis, and R_f values of twobands of YPP were similar to that of two bands of serum. Five and seven injectionswith HCG+CPG were given to female ells of control group, females showed （11.94±2.83）% and （46.04±6.22）% respectively in GSI, （0.665±0.431） ng/ml and （0.411±0.010） ng/ml in serum 17β-oestradiol （17β-E₂） levels, （4.602±3.442） ng/ml and（3.817±1.399） ng/ml in serum testosterone （T） levels. Five and seven injectionswith HCG+CPG+YPP were given to females of experimental group, GSI reached to（16.53±6.67）% and （53.23±11.25）% respectively, serum 17β-E₂ were（1.036±0.316） ng/ml and （0.411±0.068） ng/ml, serum T were （11.170±5.818） ng-ml and （3.956±1.780）ng/ml. Non-injection females, GSI was （0.50±0.32）%, serum 17β-E₂ and Tlevels were undetectable. These results indicated that exogenous eel YPP+HCG+CPGmixed injection showed significant stimulation on ovarian development and matura-tion.更多还原